Review



granulysin  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    R&D Systems granulysin
    A: BCP-ALL cells in the presence (red) or absence (grey) of BLN for three days were co-cultured with freshly isolated γδ, CD4⁺ and CD8⁺ αβ T cells/ T- and B-cell frequencies in the coculture were assessed by staining for CD3 and CD19, as exemplary shown for CD8⁺ αβ T cells. B: The release of IFNγ, GrzB and Prf into supernatants of cocultures in the presence (red) or absence (grey) of BLN was assessed via ELISA after three days, and the release of <t>Gnly</t> was analyzed after seven days. Data includes 4 to 7 healthy donors and * indicates p value between 0.01-0.05, ** indicates p value between 0.01-0.001 (paired and unpaired T-test).
    Granulysin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/granulysin/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    granulysin - by Bioz Stars, 2026-04
    93/100 stars

    Images

    1) Product Images from "Blinatumomab-driven T-cell activation in αβ and γδ T-cell subsets: Insights from in vitro assays§"

    Article Title: Blinatumomab-driven T-cell activation in αβ and γδ T-cell subsets: Insights from in vitro assays§

    Journal: bioRxiv

    doi: 10.1101/2025.11.17.688178

    A: BCP-ALL cells in the presence (red) or absence (grey) of BLN for three days were co-cultured with freshly isolated γδ, CD4⁺ and CD8⁺ αβ T cells/ T- and B-cell frequencies in the coculture were assessed by staining for CD3 and CD19, as exemplary shown for CD8⁺ αβ T cells. B: The release of IFNγ, GrzB and Prf into supernatants of cocultures in the presence (red) or absence (grey) of BLN was assessed via ELISA after three days, and the release of Gnly was analyzed after seven days. Data includes 4 to 7 healthy donors and * indicates p value between 0.01-0.05, ** indicates p value between 0.01-0.001 (paired and unpaired T-test).
    Figure Legend Snippet: A: BCP-ALL cells in the presence (red) or absence (grey) of BLN for three days were co-cultured with freshly isolated γδ, CD4⁺ and CD8⁺ αβ T cells/ T- and B-cell frequencies in the coculture were assessed by staining for CD3 and CD19, as exemplary shown for CD8⁺ αβ T cells. B: The release of IFNγ, GrzB and Prf into supernatants of cocultures in the presence (red) or absence (grey) of BLN was assessed via ELISA after three days, and the release of Gnly was analyzed after seven days. Data includes 4 to 7 healthy donors and * indicates p value between 0.01-0.05, ** indicates p value between 0.01-0.001 (paired and unpaired T-test).

    Techniques Used: Cell Culture, Isolation, Staining, Enzyme-linked Immunosorbent Assay



    Similar Products

    gene exp gnly hs00246266 m1  (Thermo Fisher)
    86
    Thermo Fisher gene exp gnly hs00246266 m1
    Gene Exp Gnly Hs00246266 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp gnly hs00246266 m1/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    gene exp gnly hs00246266 m1 - by Bioz Stars, 2026-04
    86/100 stars
    		  Buy from Supplier
    granulysin  (R&D Systems)
    93
    R&D Systems granulysin
    A: BCP-ALL cells in the presence (red) or absence (grey) of BLN for three days were co-cultured with freshly isolated γδ, CD4⁺ and CD8⁺ αβ T cells/ T- and B-cell frequencies in the coculture were assessed by staining for CD3 and CD19, as exemplary shown for CD8⁺ αβ T cells. B: The release of IFNγ, GrzB and Prf into supernatants of cocultures in the presence (red) or absence (grey) of BLN was assessed via ELISA after three days, and the release of <t>Gnly</t> was analyzed after seven days. Data includes 4 to 7 healthy donors and * indicates p value between 0.01-0.05, ** indicates p value between 0.01-0.001 (paired and unpaired T-test).
    Granulysin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/granulysin/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    granulysin - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier
    af700granulysin  (Novus Biologicals)
    93
    Novus Biologicals af700granulysin
    A: BCP-ALL cells in the presence (red) or absence (grey) of BLN for three days were co-cultured with freshly isolated γδ, CD4⁺ and CD8⁺ αβ T cells/ T- and B-cell frequencies in the coculture were assessed by staining for CD3 and CD19, as exemplary shown for CD8⁺ αβ T cells. B: The release of IFNγ, GrzB and Prf into supernatants of cocultures in the presence (red) or absence (grey) of BLN was assessed via ELISA after three days, and the release of <t>Gnly</t> was analyzed after seven days. Data includes 4 to 7 healthy donors and * indicates p value between 0.01-0.05, ** indicates p value between 0.01-0.001 (paired and unpaired T-test).
    Af700granulysin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/af700granulysin/product/Novus Biologicals
    Average 93 stars, based on 1 article reviews
    af700granulysin - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier
    af700 granulysin  (Novus Biologicals)
    93
    Novus Biologicals af700 granulysin
    A: BCP-ALL cells in the presence (red) or absence (grey) of BLN for three days were co-cultured with freshly isolated γδ, CD4⁺ and CD8⁺ αβ T cells/ T- and B-cell frequencies in the coculture were assessed by staining for CD3 and CD19, as exemplary shown for CD8⁺ αβ T cells. B: The release of IFNγ, GrzB and Prf into supernatants of cocultures in the presence (red) or absence (grey) of BLN was assessed via ELISA after three days, and the release of <t>Gnly</t> was analyzed after seven days. Data includes 4 to 7 healthy donors and * indicates p value between 0.01-0.05, ** indicates p value between 0.01-0.001 (paired and unpaired T-test).
    Af700 Granulysin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/af700 granulysin/product/Novus Biologicals
    Average 93 stars, based on 1 article reviews
    af700 granulysin - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier
    anti-human granulysin (gnly) antibody  (Becton Dickinson)
    90
    Becton Dickinson anti-human granulysin (gnly) antibody
    A: BCP-ALL cells in the presence (red) or absence (grey) of BLN for three days were co-cultured with freshly isolated γδ, CD4⁺ and CD8⁺ αβ T cells/ T- and B-cell frequencies in the coculture were assessed by staining for CD3 and CD19, as exemplary shown for CD8⁺ αβ T cells. B: The release of IFNγ, GrzB and Prf into supernatants of cocultures in the presence (red) or absence (grey) of BLN was assessed via ELISA after three days, and the release of <t>Gnly</t> was analyzed after seven days. Data includes 4 to 7 healthy donors and * indicates p value between 0.01-0.05, ** indicates p value between 0.01-0.001 (paired and unpaired T-test).
    Anti Human Granulysin (Gnly) Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti-human granulysin (gnly) antibody/product/Becton Dickinson
    Average 90 stars, based on 1 article reviews
    anti-human granulysin (gnly) antibody - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    granulysin-pe  (Thermo Fisher)
    90
    Thermo Fisher granulysin-pe
    Coculture of sorted T-cell populations and Mtb -infected macrophages: release of effector molecules <xref ref-type= a " width="250" height="auto" />
    Granulysin Pe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/granulysin-pe/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    granulysin-pe - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    granulysin  (R&D Systems Hematology)
    90
    R&D Systems Hematology granulysin
    Coculture of sorted T-cell populations and Mtb -infected macrophages: release of effector molecules <xref ref-type= a " width="250" height="auto" />
    Granulysin, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/granulysin/product/R&D Systems Hematology
    Average 90 stars, based on 1 article reviews
    granulysin - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    granulysin-pe dh2  (Thermo Fisher)
    90
    Thermo Fisher granulysin-pe dh2
    Coculture of sorted T-cell populations and Mtb -infected macrophages: release of effector molecules <xref ref-type= a " width="250" height="auto" />
    Granulysin Pe Dh2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/granulysin-pe dh2/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    granulysin-pe dh2 - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A: BCP-ALL cells in the presence (red) or absence (grey) of BLN for three days were co-cultured with freshly isolated γδ, CD4⁺ and CD8⁺ αβ T cells/ T- and B-cell frequencies in the coculture were assessed by staining for CD3 and CD19, as exemplary shown for CD8⁺ αβ T cells. B: The release of IFNγ, GrzB and Prf into supernatants of cocultures in the presence (red) or absence (grey) of BLN was assessed via ELISA after three days, and the release of Gnly was analyzed after seven days. Data includes 4 to 7 healthy donors and * indicates p value between 0.01-0.05, ** indicates p value between 0.01-0.001 (paired and unpaired T-test).

    Journal: bioRxiv

    Article Title: Blinatumomab-driven T-cell activation in αβ and γδ T-cell subsets: Insights from in vitro assays§

    doi: 10.1101/2025.11.17.688178

    Figure Lengend Snippet: A: BCP-ALL cells in the presence (red) or absence (grey) of BLN for three days were co-cultured with freshly isolated γδ, CD4⁺ and CD8⁺ αβ T cells/ T- and B-cell frequencies in the coculture were assessed by staining for CD3 and CD19, as exemplary shown for CD8⁺ αβ T cells. B: The release of IFNγ, GrzB and Prf into supernatants of cocultures in the presence (red) or absence (grey) of BLN was assessed via ELISA after three days, and the release of Gnly was analyzed after seven days. Data includes 4 to 7 healthy donors and * indicates p value between 0.01-0.05, ** indicates p value between 0.01-0.001 (paired and unpaired T-test).

    Article Snippet: Cell culture supernatants were analyzed for Interferon-γ (IFNγ; DIF50C), Granzyme B (GrzB; DGZB00), Perforin (QK8011), Granulysin (Gnly; DY3138), Perforin (Prf; QK8011) and soluble Fas-Ligand (sFAS-L; DFL00B) using sandwich ELISA kits from R&D Systems, Minneapolis, USA according to the manufacturer’s instructions.

    Techniques: Cell Culture, Isolation, Staining, Enzyme-linked Immunosorbent Assay

    Coculture of sorted T-cell populations and Mtb -infected macrophages: release of effector molecules <xref ref-type= a " width="100%" height="100%">

    Journal: Infection and Immunity

    Article Title: Polycytotoxic T cells mediate antimicrobial activity against intracellular Mycobacterium tuberculosis

    doi: 10.1128/iai.00297-24

    Figure Lengend Snippet: Coculture of sorted T-cell populations and Mtb -infected macrophages: release of effector molecules a

    Article Snippet: The following antibodies were used for flow cytometry: CD3-PerCP (clone SK7, BD Biosciences), CD8-BV605 (clone RPA-T8, BioLegend), NKG2A-FITC (clone REA 110, Miltenyi), NKG2A-PE (clone REA 110, Miltenyi), NKG2C-APC (clone REA 205, Miltenyi), granzyme B-Pacific Blue (clone GB11, BioLegend), GZMB-APC (clone GRB05, Invitrogen), perforin-PE-Cy7 (cloneB-D48, BioLegend), granulysin-Alexa488 (cloneRB1, BD Biosciences), granulysin-PE (cloneDH2, eBioscience), and CD107a-PE (BD Biosciences).

    Techniques: Infection

    NKG2C + and NKG2A + T-lymphocytes inhibit the growth of intracellular Mtb and contain a higher frequency of P-CTLs. ( A ) Macrophages were infected with Mtb , coated with an anti-CD3 antibody, and cocultured with sorted T-cell subsets for 24 h. Lysates were plated in serial dilutions and the number of CFU was determined after 14 days of incubation. The number of CFU was determined and related to the number of CFU in the culture without T cells (defined as “1.0”). The graph shows the individual values of 13 independent donors (horizontal line: median). Statistical analysis was performed using the Wilcoxon test, two-tailed. ( B ) PBMCs from 30 donors were stained for CD3, CD8, NKG2A, NKG2C, granzyme B, perforin, granulysin, and Live/Dead and analyzed by flow cytometry. The Boolean gating function in FlowJo was used to determine combinatorial expression of granzyme B, perforin, and granulysin within all subsets. The dot plot illustrates the identification of P-CTLs using a subsequent gating strategy. The full gating strategy is depicted in S3. ( C ) The figure shows the frequency of P-CTL in all 30 donors investigated. Statistical comparison was performed with the Wilcoxon test, two-tailed. ( D ) Heatmap presenting the correlation between CTL-subsets and n -fold Mtb growth using Spearman’s rank correlation test ( n = 12). R -values are depicted in the squares. CFU, colony-forming units; Mtb , Mycobacterium tuberculosis , P-CTL, polycytotoxic T-lymphocytes; PBMC, peripheral blood mononuclear cell.

    Journal: Infection and Immunity

    Article Title: Polycytotoxic T cells mediate antimicrobial activity against intracellular Mycobacterium tuberculosis

    doi: 10.1128/iai.00297-24

    Figure Lengend Snippet: NKG2C + and NKG2A + T-lymphocytes inhibit the growth of intracellular Mtb and contain a higher frequency of P-CTLs. ( A ) Macrophages were infected with Mtb , coated with an anti-CD3 antibody, and cocultured with sorted T-cell subsets for 24 h. Lysates were plated in serial dilutions and the number of CFU was determined after 14 days of incubation. The number of CFU was determined and related to the number of CFU in the culture without T cells (defined as “1.0”). The graph shows the individual values of 13 independent donors (horizontal line: median). Statistical analysis was performed using the Wilcoxon test, two-tailed. ( B ) PBMCs from 30 donors were stained for CD3, CD8, NKG2A, NKG2C, granzyme B, perforin, granulysin, and Live/Dead and analyzed by flow cytometry. The Boolean gating function in FlowJo was used to determine combinatorial expression of granzyme B, perforin, and granulysin within all subsets. The dot plot illustrates the identification of P-CTLs using a subsequent gating strategy. The full gating strategy is depicted in S3. ( C ) The figure shows the frequency of P-CTL in all 30 donors investigated. Statistical comparison was performed with the Wilcoxon test, two-tailed. ( D ) Heatmap presenting the correlation between CTL-subsets and n -fold Mtb growth using Spearman’s rank correlation test ( n = 12). R -values are depicted in the squares. CFU, colony-forming units; Mtb , Mycobacterium tuberculosis , P-CTL, polycytotoxic T-lymphocytes; PBMC, peripheral blood mononuclear cell.

    Article Snippet: The following antibodies were used for flow cytometry: CD3-PerCP (clone SK7, BD Biosciences), CD8-BV605 (clone RPA-T8, BioLegend), NKG2A-FITC (clone REA 110, Miltenyi), NKG2A-PE (clone REA 110, Miltenyi), NKG2C-APC (clone REA 205, Miltenyi), granzyme B-Pacific Blue (clone GB11, BioLegend), GZMB-APC (clone GRB05, Invitrogen), perforin-PE-Cy7 (cloneB-D48, BioLegend), granulysin-Alexa488 (cloneRB1, BD Biosciences), granulysin-PE (cloneDH2, eBioscience), and CD107a-PE (BD Biosciences).

    Techniques: Infection, Incubation, Two Tailed Test, Staining, Flow Cytometry, Expressing, Comparison

    Classification of CD3 + CD8 + CTL-subsets <xref ref-type= a " width="100%" height="100%">

    Journal: Infection and Immunity

    Article Title: Polycytotoxic T cells mediate antimicrobial activity against intracellular Mycobacterium tuberculosis

    doi: 10.1128/iai.00297-24

    Figure Lengend Snippet: Classification of CD3 + CD8 + CTL-subsets a

    Article Snippet: The following antibodies were used for flow cytometry: CD3-PerCP (clone SK7, BD Biosciences), CD8-BV605 (clone RPA-T8, BioLegend), NKG2A-FITC (clone REA 110, Miltenyi), NKG2A-PE (clone REA 110, Miltenyi), NKG2C-APC (clone REA 205, Miltenyi), granzyme B-Pacific Blue (clone GB11, BioLegend), GZMB-APC (clone GRB05, Invitrogen), perforin-PE-Cy7 (cloneB-D48, BioLegend), granulysin-Alexa488 (cloneRB1, BD Biosciences), granulysin-PE (cloneDH2, eBioscience), and CD107a-PE (BD Biosciences).

    Techniques: